A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco.
Identifieur interne : 000303 ( Main/Exploration ); précédent : 000302; suivant : 000304A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco.
Auteurs : M Farooq Hussain Munis [République populaire de Chine] ; Lili Tu ; Fenglin Deng ; Jiafu Tan ; Li Xu ; Shicheng Xu ; Lu Long ; Xianlong ZhangSource :
- Biochemical and biophysical research communications [ 1090-2104 ] ; 2010.
Descripteurs français
- KwdFr :
- Fibre de coton (MeSH), Gossypium (génétique), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Paroi cellulaire (génétique), Paroi cellulaire (physiologie), Protéines végétales (génétique), Salinité (MeSH), Stress oxydatif (MeSH), Sécheresses (MeSH), Tabac (croissance et développement), Tabac (génétique), Tabac (microbiologie), Verticillium (MeSH), Végétaux génétiquement modifiés (croissance et développement), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (microbiologie).
- MESH :
- croissance et développement : Tabac, Végétaux génétiquement modifiés.
- génétique : Gossypium, Maladies des plantes, Paroi cellulaire, Protéines végétales, Tabac, Végétaux génétiquement modifiés.
- microbiologie : Maladies des plantes, Tabac, Végétaux génétiquement modifiés.
- physiologie : Paroi cellulaire.
- Fibre de coton, Salinité, Stress oxydatif, Sécheresses, Verticillium.
English descriptors
- KwdEn :
- Cell Wall (genetics), Cell Wall (physiology), Cotton Fiber (MeSH), Droughts (MeSH), Gossypium (genetics), Oxidative Stress (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plants, Genetically Modified (genetics), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (microbiology), Salinity (MeSH), Tobacco (genetics), Tobacco (growth & development), Tobacco (microbiology), Verticillium (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- genetics : Cell Wall, Gossypium, Plant Diseases, Plants, Genetically Modified, Tobacco.
- growth & development : Plants, Genetically Modified, Tobacco.
- microbiology : Plant Diseases, Plants, Genetically Modified, Tobacco.
- physiology : Cell Wall.
- Cotton Fiber, Droughts, Oxidative Stress, Salinity, Verticillium.
Abstract
For the first time, a sea-island cotton (Gossypium barbadense L.) thaumatin-like protein gene (GbTLP1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. The presence of the transgene was verified by Southern blotting and higher expression levels of GbTLP1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. Transgenic plants with constitutively higher expression of the GbTLP1 showed enhanced resistance against different stress agents, particularly, its performance against Verticillium dahliae was exceptional. Transgenic tobacco plants also exhibited considerable resistance against Fusarium oxysporum and some abiotic stresses including salinity and drought. In this experiment, transgenic plants without GbTLP1 expression were also used as controls, which behaved similar to non-transgenic control plants. Overexpression of GbTLP1 had no significant deleterious effect on plant growth except that flowering was delayed for 3-5 weeks. The apparent pleiotropic effect of this novel gene has given us insight to the plant defense mechanism.
DOI: 10.1016/j.bbrc.2010.01.069
PubMed: 20097164
Affiliations:
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Zhang</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="eISSN">1090-2104</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (genetics)</term><term>Cell Wall (physiology)</term><term>Cotton Fiber (MeSH)</term><term>Droughts (MeSH)</term><term>Gossypium (genetics)</term><term>Oxidative Stress (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (microbiology)</term><term>Salinity (MeSH)</term><term>Tobacco (genetics)</term><term>Tobacco (growth & development)</term><term>Tobacco (microbiology)</term><term>Verticillium (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fibre de coton (MeSH)</term><term>Gossypium (génétique)</term><term>Maladies des plantes (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Paroi cellulaire (génétique)</term><term>Paroi cellulaire (physiologie)</term><term>Protéines végétales (génétique)</term><term>Salinité (MeSH)</term><term>Stress oxydatif (MeSH)</term><term>Sécheresses (MeSH)</term><term>Tabac (croissance et développement)</term><term>Tabac (génétique)</term><term>Tabac (microbiologie)</term><term>Verticillium (MeSH)</term><term>Végétaux génétiquement modifiés (croissance et développement)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Wall</term><term>Gossypium</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Gossypium</term><term>Maladies des plantes</term><term>Paroi cellulaire</term><term>Protéines végétales</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Paroi cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Wall</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cotton Fiber</term><term>Droughts</term><term>Oxidative Stress</term><term>Salinity</term><term>Verticillium</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fibre de coton</term><term>Salinité</term><term>Stress oxydatif</term><term>Sécheresses</term><term>Verticillium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">For the first time, a sea-island cotton (Gossypium barbadense L.) thaumatin-like protein gene (GbTLP1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. The presence of the transgene was verified by Southern blotting and higher expression levels of GbTLP1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. Transgenic plants with constitutively higher expression of the GbTLP1 showed enhanced resistance against different stress agents, particularly, its performance against Verticillium dahliae was exceptional. Transgenic tobacco plants also exhibited considerable resistance against Fusarium oxysporum and some abiotic stresses including salinity and drought. In this experiment, transgenic plants without GbTLP1 expression were also used as controls, which behaved similar to non-transgenic control plants. Overexpression of GbTLP1 had no significant deleterious effect on plant growth except that flowering was delayed for 3-5 weeks. The apparent pleiotropic effect of this novel gene has given us insight to the plant defense mechanism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20097164</PMID><DateCompleted><Year>2010</Year><Month>03</Month><Day>18</Day></DateCompleted><DateRevised><Year>2010</Year><Month>03</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2104</ISSN><JournalIssue CitedMedium="Internet"><Volume>393</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Feb</Month><Day>26</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco.</ArticleTitle><Pagination><MedlinePgn>38-44</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bbrc.2010.01.069</ELocationID><Abstract><AbstractText>For the first time, a sea-island cotton (Gossypium barbadense L.) thaumatin-like protein gene (GbTLP1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. The presence of the transgene was verified by Southern blotting and higher expression levels of GbTLP1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. Transgenic plants with constitutively higher expression of the GbTLP1 showed enhanced resistance against different stress agents, particularly, its performance against Verticillium dahliae was exceptional. Transgenic tobacco plants also exhibited considerable resistance against Fusarium oxysporum and some abiotic stresses including salinity and drought. In this experiment, transgenic plants without GbTLP1 expression were also used as controls, which behaved similar to non-transgenic control plants. Overexpression of GbTLP1 had no significant deleterious effect on plant growth except that flowering was delayed for 3-5 weeks. The apparent pleiotropic effect of this novel gene has given us insight to the plant defense mechanism.</AbstractText><CopyrightInformation>Copyright 2010 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Munis</LastName><ForeName>M Farooq Hussain</ForeName><Initials>MF</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tu</LastName><ForeName>Lili</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Fenglin</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Tan</LastName><ForeName>Jiafu</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Li</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Shicheng</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Long</LastName><ForeName>Lu</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xianlong</ForeName><Initials>X</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>01</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>53850-34-3</RegistryNumber><NameOfSubstance UI="C003427">thaumatin protein, plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032561" MajorTopicYN="N">Cotton Fiber</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055864" MajorTopicYN="N">Droughts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003368" MajorTopicYN="N">Gossypium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054712" MajorTopicYN="N">Salinity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020172" MajorTopicYN="Y">Verticillium</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>01</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>01</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>3</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20097164</ArticleId><ArticleId IdType="pii">S0006-291X(10)00118-X</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2010.01.069</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Deng, Fenglin" sort="Deng, Fenglin" uniqKey="Deng F" first="Fenglin" last="Deng">Fenglin Deng</name><name sortKey="Long, Lu" sort="Long, Lu" uniqKey="Long L" first="Lu" last="Long">Lu Long</name><name sortKey="Tan, Jiafu" sort="Tan, Jiafu" uniqKey="Tan J" first="Jiafu" last="Tan">Jiafu Tan</name><name sortKey="Tu, Lili" sort="Tu, Lili" uniqKey="Tu L" first="Lili" last="Tu">Lili Tu</name><name sortKey="Xu, Li" sort="Xu, Li" uniqKey="Xu L" first="Li" last="Xu">Li Xu</name><name sortKey="Xu, Shicheng" sort="Xu, Shicheng" uniqKey="Xu S" first="Shicheng" last="Xu">Shicheng Xu</name><name sortKey="Zhang, Xianlong" sort="Zhang, Xianlong" uniqKey="Zhang X" first="Xianlong" last="Zhang">Xianlong Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Munis, M Farooq Hussain" sort="Munis, M Farooq Hussain" uniqKey="Munis M" first="M Farooq Hussain" last="Munis">M Farooq Hussain Munis</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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